Agricultural Pest Control Group----Chinese Academy of Sciences ,South China Botanical Garden

I. Research Programs

A. Interaction of host plant and plant-parasitic nematodes (PPN)

B. Diversity of soil nematodes and its ecological function

C. Biological characteristics of Entomopathogenic nematode (EPN) and biological control of crop pests

II. Research Interests and objectives

A. Plant-parasitic nematodes (PPN) cause substantial agricultural loss of more than $150 billion annually throughout the world. Root-knot nematodes (RKN) and cyst nematodes are the two most damaging groups PPN and are widely distributed worldwide on many crops. Our research focuses on the two nematodes to study the interaction between nematodes and plants in the molecular and genetic level. Research includes a defined combination of themes selected among the areas of nematode–plant interaction, host-perception genes in nematodes, host defense genes in plants, resistance genes in plants, avirulence genes in nematodes, plant genetic resistance, nematode identification, nematode-disease complex infection, IPM, and biotechnology. Functional genomics study on soybean cyst nematodes is one of my important research areas, which will allow us to address genome-wide disease responses. Research discovery in this area will result in a better understanding of biology of complex interactions between plants and nematodes and correspondingly new targets for control strategies will be explored.

B. Soil nematodes are part of a complex soil ecosystem, and they are diverse and widely distribute over the world. Soil nematodes play important role in soil food web, and directly or indirectly involve in nutrition cycle, energy flow and other biological processes, etc. Not only can soil nematode diversity indicate the soil biological diversity and disturbance of soil environment, but soil nematode community structure can reflect the character of soil food web and function of soil ecosystems. If the function of soil nematodes and condition of soil ecosystems are combined, the influence of outer disturbance or environment change on soil biological diversity, soil food web and function of soil ecosystems will be further revealed.

C. Entomopathogenic nematodes (EPNs), not same as plant-parasitic nematodes, is beneficial nematodes to human and environment. EPNs as alternatives to chemical pesticiedes are used as bio-control agent to host insect. Together with symbiotic bacteria, EPN can kill insect pests. The complex of interaction among EPN, symbiotic bacteria, host insect, host-habitat plants and/or soil environment affects biological control efficiency. Therefore, our study focuses on how and why the interaction of EPN-host habitat plant, EPN-host insect, symbiotic bacteria-insect, and soil environment-EPN attraction/repulsion or EPN infection affect control efficiency. Meanwhile, we are trying to utilize EPN to control PPN. The ultimate aim is to reduce or eliminate toxic chemical control and to increase EPN control in order to protect environment and food safety.

III. Lab faculty and staff

		Name	Title	Degree	Majors
1	Leader	Congli Wang	Professor	Ph.D	Plant Pathology
2	Member	Chunjie Li	Assoicate Professor	Ph.D	Plant Protection
3	Member	Fengjuan Pan	Associate Professor	Ph.D	Ecology
4	Member	Yanfeng Hu	Assistant Professor	Ph.D	Plant Science

IV Projects in recent three years

No.	Project title	Foundation	Principal investigator	Time period
1	Jasmonic acid and Salicylic acid triggering the plant denfese against soybean cyst nematode (Heterodera glycines) in soybeans	Youth Science Foundation of Heilongjiang Province	Yanfeng Hu	2015.07-2018.07
2	Mechanism of soybean cyst nematode attraction to plant signals the pre-parasitic stage	National Natural Science Foundation of China	Congli Wang	2015.1-2018.12
3	Studies on the attractiveness of soybean root exudates to the soybean cyst nematode (Heterodera glycines)	Open Funds of Key Laboratory of Mollisols Agroecology of Chinese Academy of Sciences	Yanfeng Hu	2014.09-2017.08
4	Molecular mechanism of interaction of soybean cyst nematode and plant		Congli Wang	2013.8-2016.7
5	Screening of fungal agent promoting decomposition of straw	Black Soil Special Project for Youth	Chunjie Li	2013.1-2015.12
6	Function of bacterivores in organic matter decomposition and nitrogen mineralization	Black Soil Special Project for Youth	Fengjuan Pan	2013.1-2015.12
7	Effect of coupling water with fertilizer on soil nematode community structure and diversity	National Natural Science Foundation of China	Fengjuan Pan	2011.1-2013.12
8	Low temperature storage and biological property of entomopathogenic nematodes from cold region	Youth Science Foundation of Heilongjiang Province	Chunjie Li	2011.1-2012.12
9	Effect of land use and fertilizer on soil nematode community structure and diversity	Innovative Project of Science and Technology of Harbin	Fengjuan Pan	2011.1-2012.12

V Research achievements (Publications)

Congli Wang
1.      C Li, Y Wang, Y Hu, C Hua and C Wang*. 2015. Three dimensional study of wounded plant roots recruiting entomopathogenic nematodes with Pluronic gel as a medium. Biological Control 89:68-74.

2.      S Chen, F Pan, C Zhou, C Li, C Hua, Y Mao, Y Hu, Z Tian, X Jiao and C Wang*. 2015. Genetic variation of soybean cyst nematode races in Anda Area of Heilongjiang Province. Soil and Crop 4:42-47. (In Chinese)

3.      C Wang* and PA Roberts. 2014. Novel resistance of root-knot nematode via transgressive resistance in cotton. In: Liao et al. (Eds) Nematology Research in China, (Vol. 5.) Chinese Agricultural Scientific and Technological Press, pp273-280.

4.      SL Fudli, C Wang and VM Williamson. 2013. Ethylene signaling pathway modulates attractiveness of host roots to the root-knot nematode Meloidogyne hapla. Molecular Plant-Microbe Interactions (MPMI), 26:75-86.

5.      C Wang, M Ulloa, TR Mullens and PA Roberts. 2012. QTL analysis for transgressive resistance to root-knot nematode in interspecific cotton (Gossypium spp.) progeny derived from interspecific susceptible parents. Plos One, 7:e34874

6.      M Ulloa, C Wang, RB Hutmacher, SD Wright, MR Davis, CA Saski and PA Roberts. 2011. Mapping Fusarium wilt (FOV) race 1 resistance genes by inheritance, QTL, and sequencing composition in cotton. Molecular Genetics and Genomics, 286:21-36.

7.      C Wang, S Lower and VM Williamson. 2010. Root-Knot Nematodes Exhibit Strain-Specific Clumping Behavior That Is Inherited as a Simple Genetic Trait.PLoS ONE 5(12):e15148.doi:10.1371/journal.pone.0015148.

8.      M Ulloa, C Wang and PA Roberts. 2010. Gene action analysis by inheritance and quantitative trait loci mapping of resistance to root-knot nematodes in cotton. Plant breeding 129:541-550.

9.      C Wang, GE Bruening and VM Valerie. 2009. Determination of preferred pH for root-knot nematode aggregation using pluronic F-127 Gel. Journal of Chemical Ecology, 10, 1242-1251.

10. C Wang, S Lower and VM Valerie. 2009. Application of pluronic gel in plant-nematode interaction. Nematology 11, 453-464. (cover page)

11. C Wang, M Ulloa and PA Roberts. 2008. A transgressive segregation factor (RKN2) in Gossypium barbadense for nematode resistance clusters with gene rkn1 in G. hirsutum. Molecular Genetics and Genomics, 279: 41-52.

12. C Niu, DJ Hinchliffe, RG Cantrell, C Wang, PA Roberts, and J Zhang 2007. Identification of molecular markers associated with root-knot nematode resistance in upland cotton. Crop Science, 47:951-960.

13. C Wang, M Ulloa and PA Roberts. 2006. Identification and Mapping of Microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in Acala NemX cotton (Gossypium hirsutum L.). Theoretical & Applied Genetics, 112: 770-777.

14. C Wang and PA Roberts. 2006. Development of AFLP and derived CAPS markers for root-knot nematode resistance in cotton. Euphytica, 152: 185-196.

15. C Wang and PA Roberts. 2006. A Fusarium wilt resistance gene in Gossypium barbadense and its effect on root-knot nematode-wiltdisease complex. Phytopatholgy 96:727-734.

16. C Wang, WC Matthews and PA Roberts. 2006. Phenotypic expression of rkn1-mediated Meloidogyne incognita resistance in Gossypium hirsutum populations. Journal of Nematology, 38: 250-257.

17. C Wang, M Ulloa, PA Roberts. 2014. QTL mapping Fusarium wilt race 1 and race 4 resistance in an interspecific cotton cross between Gosypium barbadense and G. hirsutum. Phytopathology 104: 126.
18. C Li, Y Wang, F Pan, and C Wang. 2014. Pluronic gel, a useful medium to study entomopathogenic nematode (EPN) host habitat finding behavior in response to environmental cues from plant. Journal of Nematology 46: 192-193.
19. C Wang, M Ulloa, PA Roberts. 2014. Transgressive segregation of root-knot nematode resistance in cotton determined by QTL analysis. Journal of Nematology 46: 253-254.
20. C Wang, M Ulloa, PA Roberts. 2012. Genetic and physical analysis of Meloidogyne incognita resistance genes in an interspecific cotton cross Gossypium barbadense x G. hirsutum. Journal of Nematology 44: 495.
21. C Wang, M Ulloa, and PA Roberts. 2011. QTL analysis for transgressive resistance to root-knot nematode in a cotton RIL population derived from interspecific susceptible parents. Phytopathology 101: S187.
22. PA Roberts, C Wang, and M Ulloa. 2010 Genetic and physical mapping of Meloidogyne incognita resistance on chromosome 11 of Acala NemX cotton. Journal of Nematology 42: 266.
Chunjie Li
Publication
1.        Li Chunjie, Wang Yi, Hu Yanfeng, Hua Cui, Wang Congli^*. Three dimensional study of wounded plant roots recruiting entomopathogenic nematodes with Pluronic gel as a medium. Biological Control. 2015, 89, 68-74;
2.        Li Chunjie, Si Zhaosheng, Xu Yanli, Pan Fengjuan, Pan Xiangwen. Effect of Soil Leachates of Soybean Genotypes and Rotation Systems on Egg Hatch of Heterodera glycines in Northeast China. Soybean Sceince. 2015, 35: 5-11;
3.        Li Chunjie, Wang Lifang, Pan Fengjuan, Xu Yanli. Effect of acclimation ways on freezing survival of entomopathogenic nematodes. Journal of Northwest A&F University (Natural Science Edition). 2015, 43: 198-202;
4.        Li Chunjie, Sun Tao, Zhang Xingyi. Effects of Straw-decomposing Agent on Degrading Efficiency of Maize Straw and Physical and Chemical Properties of Cold and Black Soil Area in China. Acta Agriculturae Boreali-Sinica. 2015,30 (Supplement);
5.        Li Chunjie, Wang Yi, Pan Fengjiuan, Wang Congli^*. Pluronic gel, a useful medium to study entomopathogenic nematode (EPN) host habitat finding behavior in response to environmental cues from plant. Journal of Nematology. 2014,46: 192-193;
6.        Li Chunjie, Tan Guozhong, Wang Yi, Xu Yanli. Optimizing freezing storage conditions of the entomopathogenic nematode isolated from the cold region in China. Plant Protection. 2014,40: 49-54;
7.        Li Chunjie, Wang Yi, Xu Yanli, Zhang Zhiming, Meng jie, Yao Qin, Songjie. Efficacy of a mixed formulation of Heterorhabditis bacteriophora Zt (Heilongjiang isolate) and Steinernema carpocapsae All against Bradysia odoriphaga. Chinese Journal of Applied Entomology. 2013, 50: 235-241;
8.        Li Chunjie, Zhao Dan, Si Zhaosheng, Pan Fengjuan, Xu Yanli. Allelopathy effect of root diffusate from crops planted in different rotation systems on germination and growth of soybean seed. Soybean Science. 2013, 32: 206-210;
9.        Li Chunjie, Han Chenggui, Zhu Xiangming, Han Bingjin. Control efficacy of high-efficiency germicide against Cercospora leaf spot of sugar beet. Soil and Crop. 2012, 1: 186-189;
10.    Li Chunjie, Tan Guozhong, Wang Yi, Xu Yanli. Occurrence of entomopathogenic nematodes resources and overwintering conditions in Heilongjiang province. Plant Protection. 2011, 37: 120-123;
11.    Li Chunjie, Liu Hailong, Xu Yanli. Study on biological characteristics of antagonistic bacteria against soybean root rot. Soybean Science. 2011, 30: 809-812;
12.    Li Chunjie, Li Zhaolin, Song Chunyu, Zhang Xingyi. Countermeasures of continuous cropping soybean on slopping land in black soil regions of Northeast China. Soybean Sceince. 2010, 29: 474-478;
13.    Li Chunjie, Zhang Hongji, Xu Yanli, Liu Wei, Zhan Lili, Han Xinhua. Effect of biocontrol granules preparations on soybean root rot in field. System Sciences and Comprehensive Studies in Agriculture.2010, 26:330-334;
14.    Li Chunjie, Xu Yanli, Liu Hailong, Pan Fengjuan, Zhan Lili, Wang Lifang. Inhibiting effect of biocontrol bacteria on soybean pathogenic fungi. World Soybean Research Conference VIII, 36, August 10-15, 2009, Beijing, China;
15.    Li Chunjie, Xu Yanli, Wang Lifang. Freezing Survival of Entomopathogenic Nematodes Heterorhabditis sp. in Northeast China. International Symposium on the Research and Development of Entomopathogenic Nematodes, 64, April 9-12, 2009, Sanya, China;
16.    Li Chunjie, Xu Yanli, Zhao Zhiquan, Liu Jinbo. Control of soybean root rot disease with Trichoderma sp. Granules preparations. Soybean Science. 2009, 28: 499-501, 506;
17.    Li ChunJie, Xu Yanli, Tan Guozhong. Evaluations of different methods on mass production of entomopathogenic nematodes. 5^th International Congress of Nematology. 13-18 July 2008, Brisbane, Australia.230.
18.    Li Chunjie, Wang Yi, Xu Yanli, Tan Guozhong, Zhan Lili, He Hentao, Lang Lin. Laboratory screening of technology for in vivo mass production of Heterorhabditis Bacteriophora. System Sciences and Comprehensive Studies in Agriculture. 2008, 24: 398-402;
19.    Li Chunjie, Xu Yanli, Sun Yuqiu, Han Xinhua, Chen Haishan, Zhang Qian. Impacts of different terrains on continuous soybean cropping barrier. Soybean Science. 2008, 27: 629-632;
20.    Li Chunjie, Xu Yanli, Chen Haishan, Liu Hailong, Tan Guozhong, Wang Zhenyu. Effects of tillage patterns on development and yield of continuous cropping soybean. Chinese Journal of Oil Crop Sciences. 2008, 30: 455-459;
21.    Li Chunjie, Xu Yanli, Wang Yi, Liu Changzhong, Qian Xiujuan. Evaluation of effects of different methods on the quality of entomopathogenic nematodes. Plant Protection. 2007, 33: 88-92;
22.    Li Chunjie, Xu Yanli, Liu Changzhong, Si Zhaosheng, Wen Guangyue. Effect and evaluation of Entomophthora spp．on controlling Aphis glycines. Chinese journal of oil crop sciences. 2006, 6: 20-23;
23.    Li Chunjie, Xu Yanli, Liu Changzhong, Qian Xiujuan, He Zhentao, Lang Lin, Liu Hailong. Strategies of biological and ecological control for the soil-born pests in soybean. Soybean Bulletin. 2006,85: 20-23;
24.    Li Chunjie, Xu Yanli, Li Zhaolin, Shao Hongtao, Si Zhaosheng. Screening of antagonistic bacteria against pathogenic fungus of soybean root rot and their biocontrol efficiency. Soybean Science. 2004, 23: 174-177.

2）Patent：
1.   Li Chunjie, Wang Congli, Pan Fengjuan. A rapid detection method of entomopathogenic nematode recognizing insect host plant root, Patent pending number: 2013120900666740, Application date: 2013.12.09;
2. Li Chunjie, Han Bingjin, Han Chenggui. A control method of Cercospora beticola, Patent pending number: 20130045692.7, Application date: 2013.2.6;
3.   Li Chunjie. Production method of entomopathogenic nematode in vivo, Authorization Number: ZL 201210108069.7, Authorization date: 2014.2.2;
4.   Li Chunjie, Xu Yanli. A freezing storage method of Heterorhabditis Bacteriophora isolated from cold region. Authorization Number: ZL 200910071751.1, Authorization date: 2013.1.16;
5. Xu Yanli, Li Chunjie. A species of granula and its preparation method of compound anti fungi against soybean root rot. Authorization Number: ZL200610016571.X, Authorization date: 2008.10.15;
6. Xu Yanli, Li Chunjie. A mixed seeding method of controlling soybean cyst nematodes. Authorization Number: ZL200910071742.2, Authorization date: 2011.10.12.

3）Awards：
1.   Li Chunjie, Xu Yanli, Tan Guozhong, Si Zhaosheng, Han Xinhua, Bai Xiuli, Wang Lifang, Zhan Lili, Pan Fengjuan, Zhang Hongji, Zhao Dan. Project name: Low temperature storage of entomopathogenic nematode, Harbin City Science and Technology Progress Third Prize, 2013.11;
2.  Xu Yanli, Li Chunjie, Qian Xiujuan, Li Zhaolin, Xuejin, Tan Guozhong, Pan Fengjuan, Zhan Lili, Liu Jinbo, Liu Hailong, Dong Nan. Project name: Key technology study of entomopathogenic nematode mass production and application, Harbin City Science and Technology Progress Third Prize, 2007.12.

Fengjuan Pan

1.        Fengjuan Pan, Yanli Xu*, Neil B. McLaughlin, Wei liu, Xiaozeng Han, Lili Zhan, Dan Zhao. Influence of different fertilization regimes on plant-parasitic nematodes in the black soil region of Northeast China. Phytoparasitica, 2013, 41 (4): 355–363.
2.        Fengjuan Pan, Allen G. Xue*, Neil B. McLaughlin, Shuxian Li, Yanli Xu, Dan Zhao, Hongyun Qu. Colonization of Clonostachysrosea on soybean root grown in media inoculated with Fusariumgraminearum. ActaAgriculturaeScandinavica, Section B - Soil & Plant Science, 2013, 63 (6): 564-569.
3.        Fengjuan Pan, Sijia Zhang, Yan Chen, Jing Meng, Dongpo Wu, Jia Wang, Liang Xiao, Yanli Xu. Management of Entomopathogenic Nematode on Bradysia odoriphaga in Ningxia. Anhui Agricultural Science Bulletin, 2013, 19 (3): 83-86.
4.        Fengjuan Pan, Yanli Xu*, Neil B. McLaughlin, Allen G. Xue, Qing Yu, Xiaozeng Han, Wei Liu, Lili Zhan, Dan Zhao, Chunjie Li. Response of soil nematode community structure and diversity to long-term land use in the black soil region in China. Ecological Research, 2012, 27 (4): 701–714.
5.        Fengjuan Pan, Neil B. McLaughlin, Qing Yu, Allen G. Xue,Yanli Xu*,Xiaozeng Han,Chunjie Li,Dan Zhao. Responses of soil nematode community structure to different long-term fertilizer strategies in the soybean phase of a soybean-wheat-corn rotation. European Journal of Soil Biology, 2010, 46 (2): 105-111.
6.        Fengjuan Pan, Yanli Xu, Chunjie Li. Spatial distribution of soil nematodes in soybean rhizosphere under different rotation systems. System Sciences and Comprehensive Studies in Agriculture. 2009, 25(1): 33-38.
7.        Fengjuan Pan, Yanli Xu, Qing Yu, Allen G. Xue, Wei Liu. Community Structure and Seasonal Fluctuation of Soil Nematodes in Soybean Rhizosphere under Different Manure Modes. World Soybean Research Conference VIII, August 10-15, 2009, Beijing, China.
8.        Fengjuan Pan, Yanli Xu, Chunjie Li. Distribution of plant-parasitic nematodes in soybean fields under different soil type and latitude. Soybean Science, 2009, 28, (5): 869-874
9.        Fengjuan Pan, Yanli Xu, Chunjie Li, Wei Liu. Distribution of plant-parasitic nematodes in soybean fields under different soil type in Heilongjiang. Nematology Research in China, 2008, 2: 58-59.
10.    Fengjuan Pan,Yanli Xu, Chunjie Li, Xiaozeng Han, Wei Liu. Population structure of plant-parasitic nematodes under different soil type and latitude of soybean fields. 5th International Congress of Nematology, July 13-18, 2008, Brisbane, Australia.
11.    Fengjuan Pan, Yanli Xu, Chunjie Li, Xinhua Han, Wei Liu. Seasonal trend of main plant-parasitic nematodes in soybean rhizosphere under different rotation systems. Soybean Science, 2008, 27（6）: 997-1002.
12.    Fengjuan Pan, Yanli Xu. Advance on biological control to soybean cyst nematode by fungi in Chian. Soybean Bulletin, 2006, 4: 8-11.

Yanfeng Hu
1.      C Li, Y Wang, Y Hu, C Hua and C Wang. 2015. Three dimensional study of wounded plant roots recruiting entomopathogenic nematodes with Pluronic gel as a medium. Biological Control 89:68-74.
2.      Wang J, Zhang Y, Li Y, Wang X, Nan W, Hu Y, Zhang H, Zhao C, Wang F, Li P, Shi H, Bi Y (2015) Endophytic microbes Bacillus sp. LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings. Plant Cell Reports 34:1075-1087.
3.      Jia H, Hu Y, Fan T, Li J (2015) Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis. Sci Rep. doi: 10.1038/srep08251.
4.      Hu Y, You J, Liang X (2015) Nitrate reductase-mediated nitric oxide production is involved in copper tolerance in shoots of hulless barley. Plant Cell Reports 34:367-379.
5.      Liang X, Wang H, Hu Y, Mao L, Sun L, Dong T, Nan W, Bi Y (2015) Silicon does not mitigate cell death in cultured tobacco BY-2 cells subjected to salinity without ethylene emission. Plant Cell Reports 34:331-343.
6.      Hu Y, Li J, Yang L, Yang L, Nan W, Cao X, Bi Y (2014) Inhibition of root growth by narciclasine is caused by DNA damage-Induced cell cycle arrest in Lettuce seedlings. Protoplasma 251:1113-1124.
7.      Nan W, Wang X, Yang L, Hu Y, Wei Y, Liang X, Mao L, Bi Y (2014) Cyclic GMP is involved in auxin signaling during Arabidopsis root growth and development. J Exp Bot 65:1571-1583.
8.      Hu Y, Na X, Yang L, Nan W, Liu X, Zhang Y, Li J, Bi Y (2013) Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings. J Plant Physiol 170: 965-975.
9.      Liu J, Wang X, Hu Y, Hu W, Bi Y (2013) Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots. Plant Cell Rep 32: 415-429.
10. Hu Y, Yang L, Na X, You J, Hu W, Liang X, Liu J, Mao L, Wang X, Wang H, Bi Y (2012) Narciclasine inhibits the responses of Arabidopsis roots to auxin. Planta 236: 597-612.
11. Liang X, Wang H, Mao L, Hu Y, Dong T, Zhang Y, Wang X, Bi Y (2012) Involvement of COP1 in ethylene- and light-regulated hypocotyl elongation. Planta 236:1791-1802.
12. Gong H, Chen G, Li F, Wang X, Hu Y, Bi Y (2012) Involvement of G6PDH in regulation of heat stress tolerance in the calli from Przewalskia tangutica and tobacco. Biologia Plant 56:422-430.
13. Na X, Hu Y, Yue K, Lu H, Jia P, Wang H, Wang X, Bi Y (2011) Narciclasine modulates polar auxin transport in Arabidopsis roots. J Plant Physiol. 168:1149-1156.
14. Na X, Hu Y, Yue K, Lu H, Jia P, Wang H, Wang X, Bi Y (2011) Concentration-dependent effects of narciclasine on cell cycle progression in Arabidopsis root tips. BMC Plant Biol 11: 184 (co-first author).


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