论文题目:

Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library

论文题目英文:

Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library

论文编号:

第一作者:

夏正俊

第一作者英文:

Xia, Z. J.

刊物名称:

Analytical Biochemistry

卷期页码:

444, 38-40

发表年度:

2014

联系作者:

夏正俊

收录类别:

影响因子:

参与作者:

全文链接:

摘要:

Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.