Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library
Update time:
2014-08-26
论文题目:
Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library
论文题目英文:
Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library
论文编号:
第一作者:
夏正俊
第一作者英文:
Xia, Z. J.
刊物名称:
Analytical Biochemistry
卷期页码:
444, 38-40
发表年度:
2014
联系作者:
夏正俊
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摘要:
Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.